Tissue microarray analysis of hormonal signaling pathways in uterine carcinosarcoma

Am J Obstet Gynecol. 2009 Apr;200(4):457.e1-5. doi: 10.1016/j.ajog.2008.12.012. Epub 2009 Feb 6.

Abstract

Objectives: To evaluate the relationship of hormone (estrogen receptor alpha, estrogen receptor beta, progesterone receptor) and growth factor receptor (insulin-like growth factor receptor, human epidermal growth factor receptor 2) expression with disease progression in uterine carcinosarcoma.

Study design: Immunohistochemistry was performed on tissue arrays using standard methodology. Differences between groups were evaluated by the Wilcoxon rank-sum test. Interactions between tumor stage and receptor expression were determined by linear trend analysis.

Results: Compared with normal endometrium, carcinosarcomas exhibited low estrogen receptor alpha and progesterone receptor expression (all P < .01), but overexpressed estrogen receptor beta (P = .02). Estrogen receptor beta expression increased in advanced stage disease (P = .02). Insulin-like growth factor receptor expression was lower in carcinosarcoma compared with normal endometrium (P = .01). Human epidermal growth factor receptor 2 expression was elevated and increased with disease progression (P < .01).

Conclusion: In uterine carcinosarcoma, estrogen receptor beta expression is elevated and increases with disease progression, whereas estrogen receptor alpha and progesterone receptor are suppressed. Human epidermal growth factor receptor 2 expression is increased, whereas insulin-like growth factor receptor is lower than in normal endometrium. These data support a potential role for estrogen receptor beta in disease progression via crosstalk with human epidermal growth factor receptor 2.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Carcinosarcoma / chemistry
  • Carcinosarcoma / metabolism*
  • Carcinosarcoma / pathology*
  • Disease Progression
  • Estrogen Receptor alpha / analysis
  • Estrogen Receptor alpha / biosynthesis*
  • Estrogen Receptor beta / analysis
  • Estrogen Receptor beta / biosynthesis*
  • Female
  • Humans
  • Microarray Analysis
  • Neoplasm Staging
  • Pilot Projects
  • Receptors, Growth Factor / analysis
  • Receptors, Growth Factor / biosynthesis*
  • Receptors, Progesterone / analysis
  • Receptors, Progesterone / biosynthesis*
  • Signal Transduction
  • Uterine Neoplasms / chemistry
  • Uterine Neoplasms / metabolism*
  • Uterine Neoplasms / pathology*

Substances

  • Estrogen Receptor alpha
  • Estrogen Receptor beta
  • Receptors, Growth Factor
  • Receptors, Progesterone