Cannabinoid type 1 receptor antagonism delays ascites formation in rats with cirrhosis

Gastroenterology. 2009 Jul;137(1):341-9. doi: 10.1053/j.gastro.2009.01.004. Epub 2009 Jan 14.

Abstract

Background & aims: Endocannabinoids contribute to hemodynamic abnormalities of cirrhosis. Whether this favors renal sodium retention and ascites formation is unknown. We determined whether cannabinoid type 1 receptor antagonism prevents sodium retention and ascites formation in preascitic cirrhotic rats.

Methods: Once renal sodium handling was impaired, rats with carbon tetrachloride-induced cirrhosis were randomized to receive either vehicle or rimonabant (3 [group 1] or 10 [group 2] mg x kg(-1) x day(-1)) for 2 weeks. Natriuresis, sodium intake, and sodium balance were measured daily. At the end of the protocol, systemic hemodynamics, renal blood flow, ascites volume, and liver fibrosis were assessed.

Results: A significant reduction in ascites formation (group 1: 54%; group 2: 10%; vehicle: 90%) and volume (group 1: 1.6 +/- 0.3 mL; group 2: 0.5 mL; vehicle: 5.5 +/- 0.8 mL) occurred in treated rats. Rimonabant significantly improved sodium balance during week 2 (group 1: 0.98 +/- 0.08 mmol; group 2: 0.7 +/- 0.08 mmol; vehicle: 3.05 +/- 0.11 mmol). Both treated groups showed lower cardiac output and higher mean arterial pressure, peripheral vascular resistance, and renal blood flow (P < .05). Liver fibrosis was reduced in group 2 by 30% (P < .05 vs vehicle). Mean arterial pressure inversely correlated with sodium balance (R = -0.61; P = .003), but not with fibrosis score.

Conclusions: Rimonabant improves sodium balance and delays decompensation in preascitic cirrhosis. This is achieved though an improvement in systemic and renal hemodynamics, although it cannot be excluded that the antifibrotic effect of the drug may play a role.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Ascites / etiology
  • Ascites / metabolism
  • Ascites / physiopathology
  • Ascites / prevention & control*
  • Blood Pressure / drug effects
  • Carbon Tetrachloride
  • Cardiac Output / drug effects
  • Diuresis / drug effects
  • Dose-Response Relationship, Drug
  • Kidney / blood supply
  • Kidney / drug effects*
  • Kidney / metabolism
  • Kidney / physiopathology
  • Liver Cirrhosis, Experimental / chemically induced
  • Liver Cirrhosis, Experimental / complications
  • Liver Cirrhosis, Experimental / drug therapy*
  • Liver Cirrhosis, Experimental / metabolism
  • Liver Cirrhosis, Experimental / physiopathology
  • Male
  • Natriuresis / drug effects
  • Piperidines / pharmacology*
  • Pyrazoles / pharmacology*
  • Rats
  • Rats, Wistar
  • Receptor, Cannabinoid, CB1 / antagonists & inhibitors*
  • Receptor, Cannabinoid, CB1 / metabolism
  • Renal Circulation / drug effects
  • Rimonabant
  • Sodium / urine
  • Sodium, Dietary / metabolism
  • Time Factors
  • Vascular Resistance / drug effects

Substances

  • Piperidines
  • Pyrazoles
  • Receptor, Cannabinoid, CB1
  • Sodium, Dietary
  • Sodium
  • Carbon Tetrachloride
  • Rimonabant