Identification of a selective thieno[2,3-c]pyridine inhibitor of COT kinase and TNF-alpha production

Bioorg Med Chem Lett. 2009 Mar 15;19(6):1722-5. doi: 10.1016/j.bmcl.2009.01.088. Epub 2009 Jan 30.

Abstract

COT (Tpl2 in mice) is a serine/threonine MAP3 kinase that regulates production of TNF-alpha and other pro-inflammatory cytokines such as IL-1beta via the ERK/MAP kinase pathway. As TNF-alpha and IL-1beta are clinically validated targets for therapeutic intervention in rheumatoid arthritis (RA), blocking COT provides a potential avenue for amelioration of disease. Herein we describe identification of a cellular active selective small molecule inhibitor of COT kinase.

MeSH terms

  • Animals
  • Arthritis, Rheumatoid / drug therapy
  • Chemistry, Pharmaceutical / methods
  • Drug Design
  • Enzyme Inhibitors / chemical synthesis*
  • Enzyme Inhibitors / pharmacology*
  • Extracellular Signal-Regulated MAP Kinases / metabolism
  • Humans
  • Hydrogen Bonding
  • Inhibitory Concentration 50
  • Interleukin-1beta / metabolism
  • Ligands
  • MAP Kinase Kinase Kinases / antagonists & inhibitors*
  • MAP Kinase Kinase Kinases / chemistry
  • Mice
  • Molecular Structure
  • Proto-Oncogene Proteins / antagonists & inhibitors*
  • Proto-Oncogene Proteins / chemistry
  • Pyridines / chemical synthesis*
  • Pyridines / pharmacology
  • Tumor Necrosis Factor-alpha / antagonists & inhibitors*
  • Tumor Necrosis Factor-alpha / chemistry
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Enzyme Inhibitors
  • Interleukin-1beta
  • Ligands
  • Proto-Oncogene Proteins
  • Pyridines
  • Tumor Necrosis Factor-alpha
  • thienopyridine
  • Extracellular Signal-Regulated MAP Kinases
  • MAP Kinase Kinase Kinases
  • MAP3K8 protein, human