Objective: To study the expression of Aurora-B in human glioma tissue and its significance.
Methods: The total RNA was extracted from 41 human glioma tissues and 11 normal brain tissues by Trizol reagent. After reverse transcription of the total RNA into cDNAs, Aurora-B mRNA expressions in these samples were detected by quantitative real-time PCR. The protein expression in these samples was detected using immunohistochemical staining.
Results: Aurora-B mRNA and protein expressions were significantly increased in glioma tissues as compared with those in normal brain tissues.
Conclusion: Aurora-B mRNA and protein show markedly higher expressions in glioma tissue, suggesting that Aurora-B may be one of the malignant biomarkers in the pathogenesis and progression of human glioma.