Induction of NF-kappa B DNA-binding activity during the G0-to-G1 transition in mouse fibroblasts

Mol Cell Biol. 1991 Oct;11(10):4943-51. doi: 10.1128/mcb.11.10.4943-4951.1991.

Abstract

A DNA-binding factor with properties of NF-kappa B and another similar activity are rapidly induced when growth-arrested BALB/c 3T3 cells are stimulated with serum growth factors. Induction of these DNA-binding activities is not inhibited by pretreatment of quiescent cells with the protein synthesis inhibitor cycloheximide. Interestingly, the major NF-kappa B-like activity is not detected in nuclear extracts of proliferating cells, and thus its expression appears to be limited to the G0-to-G1 transition in 3T3 cells. These DNA-binding activities bind many of the expected NF-kappa B target sequences, including elements in the class I major histocompatibility complex and human immunodeficiency virus enhancers, as well as a recently identified NF-kappa B binding site upstream of the c-myc gene. Furthermore, both the class I major histocompatibility complex and c-myc NF-kappa B binding sites confer inducibility on a minimal promoter in 3T3 cells stimulated with serum growth factors. The results demonstrate that NF-kappa B-like activities are immediate-early response proteins in 3T3 cells and suggest a role for these factors in the G0-to-G1 transition.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Binding Sites / genetics
  • Cell Line
  • Fibroblasts
  • G1 Phase*
  • Gene Expression Regulation / drug effects*
  • Genes, MHC Class I
  • Genes, myc
  • Growth Substances / pharmacology*
  • HIV Enhancer / genetics
  • Mice
  • Molecular Sequence Data
  • NF-kappa B / metabolism*
  • Promoter Regions, Genetic / genetics
  • Resting Phase, Cell Cycle*

Substances

  • Growth Substances
  • NF-kappa B