Abstract
Aging induces gradual yet massive cell death in higher organisms, including annual plants. Even so, the underlying regulatory mechanisms are barely known, despite the long-standing interest in this topic. Here, we demonstrate that ORE1, which is a NAC (NAM, ATAF, and CUC) transcription factor, positively regulates aging-induced cell death in Arabidopsis leaves. ORE1 expression is up-regulated concurrently with leaf aging by EIN2 but is negatively regulated by miR164. miR164 expression gradually decreases with aging through negative regulation by EIN2, which leads to the elaborate up-regulation of ORE1 expression. However, EIN2 still contributes to aging-induced cell death in the absence of ORE1. The trifurcate feed-forward pathway involving ORE1, miR164, and EIN2 provides a highly robust regulation to ensure that aging induces cell death in Arabidopsis leaves.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aging
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Apoptosis*
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Arabidopsis / cytology
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Arabidopsis / genetics
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Arabidopsis / physiology*
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / physiology*
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Down-Regulation
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Gene Expression Regulation, Plant
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Genes, Plant
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MicroRNAs / genetics
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MicroRNAs / physiology*
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Mutation
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Plant Leaves / cytology
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Plant Leaves / physiology*
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Plants, Genetically Modified
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA, Plant / genetics
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RNA, Plant / physiology*
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Receptors, Cell Surface / genetics
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Receptors, Cell Surface / physiology*
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Signal Transduction
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Transcription Factors / genetics
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Transcription Factors / physiology*
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Up-Regulation
Substances
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Arabidopsis Proteins
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EIN2 protein, Arabidopsis
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MIRN164 microRNA, Arabidopsis
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MicroRNAs
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ORE1 protein, Arabidopsis
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RNA, Messenger
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RNA, Plant
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Receptors, Cell Surface
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Transcription Factors