Isolation of a human placenta cDNA coding for a protein related to the vascular permeability factor

Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):9267-71. doi: 10.1073/pnas.88.20.9267.

Abstract

A human cDNA coding for a protein related to the vascular permeability factor (VPF) was isolated from a term placenta cDNA library; we therefore named its product placenta growth factor (PlGF). PlGF is a 149-amino-acid-long protein and is highly homologous (53% identity) to the platelet-derived growth factor-like region of human VPF. Computer analyses reveal a putative signal peptide and two probable N-glycosylation sites in the PlGF protein, one of which is also conserved in human VPF. By using N-glycosidase F, tunicamycin, and specific antibodies produced in both chicken and rabbit, we demonstrate that PlGF, derived from transfected COS-1 cells, is actually N-glycosylated and secreted into the medium. In addition, PlGF, like VPF, proves to be a dimeric protein. Finally, a conditioned medium from COS-1 cells containing PlGF is capable of stimulating specifically the growth of CPA, a line of endothelial cells, in vitro.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Division / drug effects
  • Cell Line
  • Choriocarcinoma
  • Cloning, Molecular
  • DNA / genetics*
  • DNA / isolation & purification
  • Endothelial Growth Factors / genetics*
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / drug effects
  • Female
  • Gene Library
  • Humans
  • Lymphokines / genetics*
  • Macromolecular Substances
  • Molecular Sequence Data
  • Molecular Weight
  • Oligodeoxyribonucleotides
  • Oligonucleotide Probes
  • Placenta / physiology*
  • Placenta Growth Factor
  • Pregnancy
  • Pregnancy Proteins / analysis
  • Pregnancy Proteins / genetics*
  • Pregnancy Proteins / pharmacology
  • Recombinant Proteins / analysis
  • Recombinant Proteins / pharmacology
  • Sequence Homology, Nucleic Acid
  • Transfection
  • Uterine Neoplasms
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors

Substances

  • Endothelial Growth Factors
  • Lymphokines
  • Macromolecular Substances
  • Oligodeoxyribonucleotides
  • Oligonucleotide Probes
  • PGF protein, human
  • Pregnancy Proteins
  • Recombinant Proteins
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors
  • Placenta Growth Factor
  • DNA

Associated data

  • GENBANK/S60069
  • GENBANK/S60071
  • GENBANK/S60073
  • GENBANK/S60076
  • GENBANK/S60079
  • GENBANK/S60081
  • GENBANK/S60988
  • GENBANK/S61158
  • GENBANK/S61170
  • GENBANK/X54936