Vascular endothelial growth factor-induced chemotaxis and IL-10 from T cells

Cell Immunol. 2009;256(1-2):72-8. doi: 10.1016/j.cellimm.2009.01.006. Epub 2009 Feb 26.

Abstract

Vascular endothelial growth factor (VEGF) is a proangiogenic mediator that promotes tumor growth. The role of VEGF in T lymphocytes is unknown. We found that T lymphocytes activated by either anti-CD3 monoclonal antibody (mAb) plus anti-CD28 mAb or by antigens on antigen-presenting cells transcribed mRNA for VEGF receptor 1 (VEGFR1) and VEGFR2. However, only VEGFR1 was expressed on the T cell surface. The addition of VEGF to either resting or activated T cells did not affect their proliferation, but VEGF increased IL-10 production and slightly decreased IFN-gamma production. A chemotaxis assay revealed that activated T lymphocytes migrate in response to VEGF. Our data suggest that VEGF has a direct immunomodulatory effect on T cells. Engagement of a high concentration of VEGF with VEGFR1 on T cells may cause T cells to migrate to tumor sites, and this interaction may play a role in IL-10-mediated immune evasion by tumor cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cell Line
  • Cell Proliferation / drug effects
  • Chemotaxis, Leukocyte / drug effects*
  • DNA Primers / genetics
  • Humans
  • Immunologic Factors / pharmacology*
  • Immunotherapy
  • In Vitro Techniques
  • Interferon-gamma / biosynthesis
  • Interleukin-10 / biosynthesis*
  • Lymphocyte Activation
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Models, Immunological
  • Neoplasms / immunology
  • Neoplasms / therapy
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • T-Lymphocytes / cytology
  • T-Lymphocytes / drug effects*
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / physiology
  • Vascular Endothelial Growth Factor A / pharmacology*
  • Vascular Endothelial Growth Factor Receptor-1 / genetics
  • Vascular Endothelial Growth Factor Receptor-1 / metabolism
  • Vascular Endothelial Growth Factor Receptor-2 / genetics
  • Vascular Endothelial Growth Factor Receptor-2 / metabolism

Substances

  • DNA Primers
  • Immunologic Factors
  • RNA, Messenger
  • Vascular Endothelial Growth Factor A
  • Interleukin-10
  • Interferon-gamma
  • Vascular Endothelial Growth Factor Receptor-1
  • Vascular Endothelial Growth Factor Receptor-2