Incorporation of neutral C-terminal residues in 3-amidinophenylalanine-derived matriptase inhibitors

Bioorg Med Chem Lett. 2009 Apr 1;19(7):1960-5. doi: 10.1016/j.bmcl.2009.02.047. Epub 2009 Feb 14.

Abstract

A novel series of matriptase inhibitors based on previously identified tribasic 3-amidinophenylalanine derivatives was prepared. The C-terminal basic group was replaced by neutral residues to reduce the hydrophilicity of the inhibitors. The most potent compound 22 inhibits matriptase with a K(i) value of 0.43 nM, but lacks selectivity towards factor Xa. By combination with neutral N-terminal sulfonyl residues several potent thrombin inhibitors were identified, which had reduced matriptase affinity.

MeSH terms

  • Administration, Oral
  • Amidines / chemistry*
  • Amidines / pharmacology
  • Animals
  • Factor Xa / metabolism
  • Factor Xa Inhibitors
  • Phenylalanine / analogs & derivatives
  • Phenylalanine / chemistry*
  • Phenylalanine / pharmacology
  • Rats
  • Serine Endopeptidases / chemistry*
  • Serine Endopeptidases / metabolism
  • Serine Proteinase Inhibitors / chemistry*
  • Serine Proteinase Inhibitors / pharmacology
  • Sulfonamides / chemistry*
  • Sulfonamides / pharmacology
  • Thrombin / antagonists & inhibitors
  • Thrombin / metabolism

Substances

  • Amidines
  • Factor Xa Inhibitors
  • Serine Proteinase Inhibitors
  • Sulfonamides
  • Phenylalanine
  • Serine Endopeptidases
  • matriptase
  • Thrombin
  • Factor Xa