Objective: To investigate the expression of AQP1 in endothelial cells under hypoxia conditions in vitro.
Methods: The cultured human umbilical vein endothelial cells were randomly assigned to hypoxia groups (exposed to 1% of O2 for 3 h, 6 h, 12 h and 24 h respectively), and control group (ordinary culture). The expressions of AQP1 mRNA and protein were detected by real time PCR and immunohistochemistry respectively.
Results: The expressions of AQP1 protein and mRNA increased after exposure to hypoxia initially and then decreased afterwards.
Conclusion: Hypoxia regulates the expression of AQP1 in vascular endothelial cells.