Development of a solid phase enzyme immunoassay for the detection of anti-Ro autoantibodies

Scand J Rheumatol. 1991;20(4):267-73. doi: 10.3109/03009749109096799.

Abstract

An enzyme immunoassay was developed to detect anti-Ro(SS-A) autoantibodies. Both Ro-antigen components (52 and 60 kD) were purified from a pig spleen extract, using fast protein liquid chromatography (FPLC). Anti-La, anti-RNP, anti-DNA and anti-Sm antibodies do not react to the purified antigen. There was a strong correlation between anti-Ro activity in EIA and the titers in counter immunoelectrophoresis (rs = 0.893). Anti-Ro antibodies were found in 54 (69.2%) of 78 SLE sera by the developed EIA.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Autoantibodies / analysis*
  • Autoantibodies / isolation & purification
  • Autoantigens / analysis
  • Autoantigens / chemistry
  • Autoantigens / immunology*
  • Chromatography, Liquid / methods
  • Counterimmunoelectrophoresis
  • Immunoblotting
  • Immunoenzyme Techniques*
  • Lupus Erythematosus, Systemic / immunology
  • Molecular Weight
  • RNA, Small Cytoplasmic*
  • Reference Values
  • Ribonucleoproteins*
  • Swine

Substances

  • Autoantibodies
  • Autoantigens
  • RNA, Small Cytoplasmic
  • RO60 protein, human
  • Ribonucleoproteins
  • SS-A antigen