Amino acid alterations in Gag that confer the ability to grow in simian cells on HIV-1 are located at a narrow CA region

J Med Invest. 2009 Feb;56(1-2):21-5. doi: 10.2152/jmi.56.21.

Abstract

We previously generated a prototype monkey-tropic human immunodeficiency virus type 1 (HIV-1) designated NL-DT5R. This viral clone has a small region of simian immunodeficiency virus (SIV) within Gag capsid (CA) protein and also SIV Vif protein, but displays a poor growth phenotype in simian cells. To improve the growth potential of NL-DT5R, we have constructed a series of its gag variant viruses. Out of fourteen viral clones generated, five were infectious for simian HSC-F cells, and two of the infectious variants grew similarly with NL-DT5R. Taking their genome structures into consideration, our data here clearly show that a narrow CA region within the Gag protein, i.e., the domain around cyclophilin A (CypA)-binding loop, is critical for the growth ability of HIV-1 in simian cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / analysis*
  • Animals
  • Cell Line
  • Cell Proliferation
  • Cyclophilin A / analysis*
  • Cyclophilin A / physiology
  • Disease Models, Animal
  • Epithelial Cells / virology
  • Gene Products, gag / analysis*
  • Gene Products, gag / physiology*
  • Gene Products, vif / analysis
  • Gene Products, vif / physiology
  • HIV-1 / physiology*
  • Humans
  • Macaca fascicularis
  • Molecular Sequence Data
  • Mutation
  • Simian Immunodeficiency Virus / physiology*
  • T-Lymphocytes / virology*

Substances

  • Amino Acids
  • Gene Products, gag
  • Gene Products, vif
  • Cyclophilin A