Characterization of a CD44/CD122int memory CD8 T cell subset generated under sterile inflammatory conditions

J Immunol. 2009 Mar 15;182(6):3846-54. doi: 10.4049/jimmunol.0802438.

Abstract

Most memory CD8 T cell subsets that have been hitherto defined are generated in response to infectious pathogens. In this study, we have characterized the CD8 T cells that survive priming conditions, devoid of pathogen-derived danger signals. In both a TCR-transgenic model and a model of contact hypersensitivity, we show that the priming of naive CD8 T cells under sterile inflammatory conditions generates memory. The corresponding memory CD8 T cells can be identified by their intermediate expression levels of CD44 and CD122. We also show that CD44/122(int) memory CD8 T cells spontaneously develop in wild type mice and that they display intermediate levels of several other memory traits including functional (IFN-gamma secretion capacity, CCL5 messenger stores), phenotypic, and molecular (T-bet and eomesodermin expression levels) features. We finally show that they correspond to an early differentiation stage and can further differentiate in CD44/122(high) memory T cells. Altogether, our results identify a new memory CD8 T cell subset that is generated under sterile inflammatory conditions and involved in the recall contact hypersensitivity reactions that are responsible for allergic contact dermatitis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers / metabolism
  • CD8-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / metabolism*
  • CD8-Positive T-Lymphocytes / transplantation
  • Cell Differentiation / genetics
  • Cell Differentiation / immunology*
  • Dermatitis, Contact / genetics
  • Dermatitis, Contact / immunology
  • Hyaluronan Receptors / biosynthesis
  • Hyaluronan Receptors / physiology*
  • Immunologic Memory / genetics*
  • Inflammation Mediators / metabolism
  • Inflammation Mediators / physiology*
  • Interleukin-2 Receptor beta Subunit / biosynthesis
  • Interleukin-2 Receptor beta Subunit / physiology*
  • Lymphocyte Activation / genetics
  • Lymphocyte Activation / immunology*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, Transgenic
  • T-Lymphocyte Subsets / cytology
  • T-Lymphocyte Subsets / immunology
  • T-Lymphocyte Subsets / metabolism

Substances

  • Biomarkers
  • Cd44 protein, mouse
  • Hyaluronan Receptors
  • Il2rb protein, mouse
  • Inflammation Mediators
  • Interleukin-2 Receptor beta Subunit