In the present study, the samples of landfill leachate of 0, 5, 10-year-old were respectively taken from landfill plant. Based on a modified Leenheer fractionation scheme, dissolved organic matter (DOM) extracted from landfill leachate of three different ages was fractioned according to their polarities and charge characteristics by using XAD-8 resin, and the fractions of hydrophobic acid (HOA), neutral (HON) and hydrophilic matter (HIM) were obtained, Then the fluorescence and UV spectra of DOM fractions were determined. The fluorescence synchronous scan spectra of DOM fractions exhibited a primary peak at 280 nm for 0-year-old, while the primary peak exhibited at 340nm for 5 and 10 year-old, suggesting that DOM fractions contained mainly protein-like matter at initial stage of landfill, and with the increase in landfill ages, aromatic structures of DOM fractions in leachate were enriched. Among the DOM fractions of HOA, HON and HIM at different ages of landfill leachate, the fluorescence and UV spectra all indicated that the molecular weight, content of aromatic compounds and degree of condensation were all in the order of HOA>HON>HIM. The ratio of UV absorbance at 253 nm to that at 203 nm (A253/A203) showed, that the substituent on the aromatic ring of HOA and HON fractions consisted mainly of carbonyl, carboxyl and hydroxyl; while that of HIM consisted of aliphatic chains, and the content of aromatic compounds was lower than that of HOA and HON; which implied that the HIM displayed a lower molecular weight and simpler structure compared to HOA and HON. Altogether, the results obtained from fluorescence and UV spectra indicate that the degree of aromatization increased in DOM fractions of leachate with the landfill ages, in the following order: HOA > HON > HIM.