Abstract
Sensitive and fast methods for the profiling of biologically important molecules are highly demanded. Mucins are densely O-glycosylated glycoproteins found at mucosal surfaces and are of great medical interest. Here we describe sensitive methods for the analysis of O-glycans from mucins using gel electrophoresis, and chromatography by nanoLC on graphite columns and structural analysis by electrospray mass spectrometry on a linear trap mass spectrometer.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Blotting, Western / methods
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Carbohydrate Sequence
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Chromatography, Liquid / instrumentation
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Chromatography, Liquid / methods
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Electrophoresis, Polyacrylamide Gel / methods
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Glycomics / methods
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Humans
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Molecular Sequence Data
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Mucins / analysis
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Mucins / chemistry
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Mucins / isolation & purification
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Nanotechnology / instrumentation
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Nanotechnology / methods
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Polysaccharides / analysis*
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Polysaccharides / chemistry
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Polysaccharides / isolation & purification
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Sensitivity and Specificity
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Tandem Mass Spectrometry / instrumentation
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Tandem Mass Spectrometry / methods*