Interleukin-1beta is a positive regulator of TIARP/STAMP2 gene and protein expression in adipocytes in vitro

Susan Kralisch; Grit Sommer; Sebastian Weise; Jana Lipfert; Ulrike Lossner; Manja Kamprad; Kathleen Schröck; Matthias Bluher; Michael Stumvoll; Mathias Fasshauer

doi:10.1016/j.febslet.2009.03.015

Interleukin-1beta is a positive regulator of TIARP/STAMP2 gene and protein expression in adipocytes in vitro

FEBS Lett. 2009 Apr 2;583(7):1196-200. doi: 10.1016/j.febslet.2009.03.015. Epub 2009 Mar 14.

Authors

Susan Kralisch¹, Grit Sommer, Sebastian Weise, Jana Lipfert, Ulrike Lossner, Manja Kamprad, Kathleen Schröck, Matthias Bluher, Michael Stumvoll, Mathias Fasshauer

Affiliation

¹ University of Leipzig, Department of Internal Medicine III, Leipzig, Germany.

PMID: 19289123
DOI: 10.1016/j.febslet.2009.03.015

Abstract

The impact of interleukin (IL)-1beta on tumor necrosis factor alpha-induced adipose-related protein (TIARP)/six-transmembrane protein of prostate 2 (STAMP2) was determined in adipocytes. TIARP/STAMP2 mRNA synthesis was significantly stimulated by IL-1beta in a dose- and time-dependent fashion in 3T3-L1 adipocytes. Signaling studies suggested that janus kinase 2, nuclear factor kappaB, and p44/42 mitogen-activated protein kinase are involved in IL-1beta-induced TIARP/STAMP2 mRNA expression. Furthermore, IL-1beta, TNFalpha, and IL-6 showed synergistic stimulatory effects on TIARP/STAMP2 gene expression. Moreover, both TIARP/STAMP2 mRNA synthesis and protein expression were induced by IL-1beta in fully differentiated human mesenchymal stem cell-derived adipocytes (hMSC-Ad). Taken together, TIARP/STAMP2 is highly upregulated in 3T3-L1 cells and hMSC-Ad by IL-1beta and might, therefore, modulate proinflammatory and insulin resistance-inducing effects of IL-1beta.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3T3-L1 Cells
Adipocytes / cytology
Adipocytes / metabolism*
Animals
Cell Differentiation / drug effects
Cell Differentiation / physiology
Dose-Response Relationship, Drug
Drug Synergism
Gene Expression Regulation / drug effects*
Gene Expression Regulation / physiology
Humans
Insulin Resistance / physiology
Interleukin-1beta / agonists
Interleukin-1beta / pharmacology*
Interleukin-6 / agonists
Interleukin-6 / pharmacology
Janus Kinase 2 / metabolism
Membrane Proteins / biosynthesis*
Mesenchymal Stem Cells / cytology
Mesenchymal Stem Cells / metabolism
Mice
Mitogen-Activated Protein Kinase 1 / metabolism
NF-kappa B / metabolism
Oxidoreductases / biosynthesis*
RNA, Messenger / biosynthesis
Tumor Necrosis Factor-alpha / agonists
Tumor Necrosis Factor-alpha / pharmacology

Substances

IL6 protein, human
Interleukin-1beta
Interleukin-6
Membrane Proteins
NF-kappa B
RNA, Messenger
Tiarp protein, mouse
Tumor Necrosis Factor-alpha
Oxidoreductases
STEAP4 protein, human
JAK2 protein, human
Jak2 protein, mouse
Janus Kinase 2
Mitogen-Activated Protein Kinase 1