Abstract
Chicken coccidiosis is a major parasitic disease with substantial economic burden to the poultry industry. Enhanced green fluorescent protein (EGFP) tagged recombinant Bacille Calmette-Guerin (rBCG), as a fusion protein with coccidian rhomboid antigen was constructed to track rBCG in vivo in chickens in this study. Immunization of chickens with one dose of rBCG pMV361-Rho/EGFP induced humoral immune response. The colonization of rBCG in liver, spleen, lung, kidney and caecum was observed by laser confocal microscopy. Real-time quantitative RT-PCR showed a rise expression level of rhomboid protein on the 7th day and a peak on the 14th day and disappearance on the 28th day after immunization. These results have significant implications for the development of rBCG vaccines against avian coccidiosis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Chickens / genetics
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Chickens / immunology
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Chickens / metabolism
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Coccidiosis / immunology
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Coccidiosis / prevention & control
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Coccidiosis / veterinary*
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Eimeria tenella / genetics
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Eimeria tenella / immunology*
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Escherichia coli / genetics
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Gene Expression / genetics
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Gene Expression / immunology
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Genetic Vectors / genetics
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Green Fluorescent Proteins / immunology
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Green Fluorescent Proteins / metabolism
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Immunization / methods
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Male
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Mycobacterium bovis / genetics*
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Mycobacterium bovis / immunology*
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Mycobacterium bovis / metabolism
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Poultry Diseases / genetics
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Poultry Diseases / immunology
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Poultry Diseases / prevention & control*
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Random Allocation
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Recombinant Fusion Proteins / immunology
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Recombinant Fusion Proteins / metabolism
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Specific Pathogen-Free Organisms
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Tissue Distribution
Substances
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Recombinant Fusion Proteins
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enhanced green fluorescent protein
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Green Fluorescent Proteins