The NER proteins are differentially expressed in ever smokers and in never smokers with lung adenocarcinoma

Ann Oncol. 2009 Jul;20(7):1257-63. doi: 10.1093/annonc/mdn785. Epub 2009 Mar 17.

Abstract

Background: The expression levels of excision repair cross-complementation group 1 (ERCC1), replication protein A (RPA) and xeroderma pigmentosum group F (XPF) nucleotide excision repair proteins may be important in the response to platin-based therapy in lung cancer patients. It is not known whether ERCC1, RPA and XPF expression levels differ between ever smokers (ES) and never smokers (NS).

Patients and methods: ERCC1, RPA and XPF expression levels were immunohistochemically evaluated in 125 patients with resected lung adenocarcinoma (AC) and carefully reviewed smoking status.

Results: ERCC1 was correlated with XPF (P = 0.001), but not with RPA (P = 0.11). In the univariate analysis, ERCC1 and XPF levels were higher in NS compared with ES (P = 0.004 and P = 0.003, respectively). In the multivariate analysis, the smoking status was predictive of the ERCC1 level [odds ratio (OR) 2.5, 95% confidence interval (CI) 1.03-6.2] after adjustment for variables linked to the smoking status, including age and the presence of bronchioloalveolar (BAC) features. The smoking status was also predictive of both RPA (OR 6.7, 95% CI 1.5-33.3) and XPF levels (OR 12.5, 95% CI 2.9-50) after adjusting for age, sex and BAC features.

Conclusion: In patients with resected lung AC, ERCC1, RPA and XPF expression levels are higher in NS compared with ES.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / metabolism*
  • Adenocarcinoma / pathology
  • Aged
  • Cohort Studies
  • DNA-Binding Proteins / metabolism
  • Endonucleases / metabolism
  • Female
  • Humans
  • Immunohistochemistry
  • Logistic Models
  • Lung Neoplasms / metabolism*
  • Lung Neoplasms / pathology
  • Male
  • Microarray Analysis
  • Middle Aged
  • Multivariate Analysis
  • Proportional Hazards Models
  • Replication Protein A / metabolism
  • Sex Distribution
  • Smoking / adverse effects*
  • Smoking / metabolism

Substances

  • DNA-Binding Proteins
  • Replication Protein A
  • xeroderma pigmentosum group F protein
  • ERCC1 protein, human
  • Endonucleases