Abstract
A 57 kDa protein was detected in an NIH3T3 transformant induced by retTPC, an activated form of the ret proto-oncogene which encodes a receptor-tyrosine kinase. A high phosphorylation state and increased activity of a TPA responsive element was observed in the transformant. Increased expression of c-jun mRNA was also detected. A 40 kDa protein in the retTPC transformant, which was specifically immunoprecipitable with v-jun antiserum, was highly phosphorylated mainly at a serine residue(s). These data suggest that an aberrant signal triggered by a retTPC product affects the cellular serine/threonine phosphorylation state resulting in high phosphorylation of c-jun protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Blotting, Northern
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Blotting, Western
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Cell Line, Transformed
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Chloramphenicol O-Acetyltransferase / genetics
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Chloramphenicol O-Acetyltransferase / metabolism
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Drosophila Proteins*
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Mice
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Molecular Sequence Data
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Protein-Tyrosine Kinases / genetics*
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Proto-Oncogene Proteins / analysis
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Proto-Oncogene Proteins / genetics*
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Proto-Oncogene Proteins c-jun / genetics
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Proto-Oncogene Proteins c-ret
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Proto-Oncogenes*
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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Receptor Protein-Tyrosine Kinases*
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Repetitive Sequences, Nucleic Acid
Substances
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Drosophila Proteins
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-jun
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RNA, Messenger
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Chloramphenicol O-Acetyltransferase
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Protein-Tyrosine Kinases
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Proto-Oncogene Proteins c-ret
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Receptor Protein-Tyrosine Kinases
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Ret protein, Drosophila
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Ret protein, mouse