Abstract
Op18 is a highly conserved major cytosolic phosphoprotein that is expressed at high levels in acute leukemia and in neuroblastoma. In this study we present evidence pointing to a role for Op18 in cellular proliferation. Blocking of Op18 mRNA translation using antisense oligonucleotides delayed entrance of mitotically stimulated normal peripheral blood lymphocytes into the S phase. Moreover treatment of HL-60 promyelocytic leukemia cells with DMSO or PMA which induced terminal differentiation resulted in a decrease in the level of Op18 RNA and protein. Inhibition of lymphoid proliferation with cyclosporin also resulted in reduced Op18 levels.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Cell Differentiation / drug effects
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Cell Division*
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Dimethyl Sulfoxide / pharmacology
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Humans
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Lymphocyte Activation / drug effects
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Lymphocytes / drug effects
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Lymphocytes / immunology
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Microtubule Proteins*
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Molecular Sequence Data
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Neoplasm Proteins / physiology*
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Oligonucleotides, Antisense / pharmacology
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Phosphoproteins / analysis
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Phosphoproteins / genetics
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Phosphoproteins / physiology*
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Protein Biosynthesis / drug effects
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RNA, Messenger / genetics
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Stathmin
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Tetradecanoylphorbol Acetate
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Tumor Cells, Cultured
Substances
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Microtubule Proteins
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Neoplasm Proteins
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Oligonucleotides, Antisense
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Phosphoproteins
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RNA, Messenger
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STMN1 protein, human
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Stathmin
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Tetradecanoylphorbol Acetate
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Dimethyl Sulfoxide