In vitro immunostimulatory properties of Abrus lectins derived peptides in tumor bearing mice

Phytomedicine. 2009 Aug;16(8):776-82. doi: 10.1016/j.phymed.2009.01.006. Epub 2009 Mar 20.

Abstract

In vitro immunostimulatory effect of Abrus lectins derived peptide fractions (AGP and ABP) was investigated in DL bearing mice. Both AGP and ABP were found to activate splenocytes and induced production of cytokines like IL-2, IFN-gamma and TNF-alpha indicating a Th1 type of immune response. Analysis of in vitro treated splenocytes by flow cytometry revealed an increase in percentage of T and B cell with high expression of activation markers (CD25(+) and CD71(+)). At the same time, expression of co-stimulatory markers was significantly high compared to tumor control. The tumor associated macrophages were able to stimulate NO production, IL-1 secretion, increased phagocytosis and decreased expression of mannose receptor. It was also observed that NK cell was activated by AGP and ABP. These results suggest that both AGP and ABP act as immunostimulants in vitro in DL bearing mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abrin / metabolism*
  • Abrus / chemistry*
  • Adjuvants, Immunologic / pharmacology
  • Adjuvants, Immunologic / therapeutic use*
  • Agglutinins / metabolism*
  • Animals
  • Antigens, CD / metabolism
  • Antineoplastic Agents, Phytogenic / pharmacology
  • Antineoplastic Agents, Phytogenic / therapeutic use*
  • B-Lymphocytes / drug effects
  • Cell Line, Tumor
  • Cytokines / biosynthesis
  • Cytokines / metabolism
  • Interleukin-2 Receptor alpha Subunit / metabolism
  • Killer Cells, Natural / drug effects
  • Lectins, C-Type / antagonists & inhibitors
  • Lymphoma / drug therapy*
  • Macrophages / drug effects
  • Mannose Receptor
  • Mannose-Binding Lectins / antagonists & inhibitors
  • Mice
  • Nitric Oxide / biosynthesis
  • Peptides / metabolism
  • Peptides / pharmacology
  • Peptides / therapeutic use*
  • Phagocytosis / drug effects
  • Phytotherapy
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use
  • Plants, Medicinal / chemistry
  • Receptors, Cell Surface / antagonists & inhibitors
  • Receptors, Transferrin / metabolism
  • Seeds
  • Spleen / cytology
  • Spleen / drug effects
  • T-Lymphocytes / drug effects

Substances

  • Adjuvants, Immunologic
  • Agglutinins
  • Antigens, CD
  • Antineoplastic Agents, Phytogenic
  • CD71 antigen
  • Cytokines
  • Interleukin-2 Receptor alpha Subunit
  • Lectins, C-Type
  • Mannose Receptor
  • Mannose-Binding Lectins
  • Peptides
  • Plant Extracts
  • Receptors, Cell Surface
  • Receptors, Transferrin
  • Abrin
  • Nitric Oxide