Abstract
A series of 1-aryl-3,4-dihydroisoquinoline inhibitors of JNK3 are described. Compounds 20 and 24 are the most potent inhibitors (pIC50 7.3 and 6.9, respectively in a radiometric filter binding assay), with 10- and 1000-fold selectivity over JNK2 and JNK1, respectively, and selectivity within the wider mitogen-activated protein kinase (MAPK) family against p38alpha and ERK2. X-ray crystallography of 16 reveals a highly unusual binding mode where an H-bond acceptor interaction with the hinge region is made by a chloro substituent.
MeSH terms
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Binding Sites / physiology
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Fluorescence Polarization / methods
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Humans
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Isoquinolines / chemical synthesis*
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Isoquinolines / metabolism
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Isoquinolines / pharmacology
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Mitogen-Activated Protein Kinase 10 / antagonists & inhibitors*
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Mitogen-Activated Protein Kinase 10 / metabolism
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Mitogen-Activated Protein Kinase 14 / antagonists & inhibitors
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Mitogen-Activated Protein Kinase 14 / metabolism
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Mitogen-Activated Protein Kinase 8 / antagonists & inhibitors
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Mitogen-Activated Protein Kinase 8 / metabolism
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Protein Kinase Inhibitors / chemical synthesis*
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Protein Kinase Inhibitors / metabolism
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Protein Kinase Inhibitors / pharmacology
Substances
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G 1617
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Isoquinolines
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Protein Kinase Inhibitors
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Mitogen-Activated Protein Kinase 10
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Mitogen-Activated Protein Kinase 14
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Mitogen-Activated Protein Kinase 8