Bleeding during liver surgery and liver transplantation (LT) can be caused by coagulation disorders, portal vein hypertension, and adhesions from previous surgical procedures. We describe here how we controlled life-threatening bleeding from a portal vein anastomosis during a third cadaveric LT, by using a carrier-bound sealant (TachoSil). This technique proved effective to control severe anastomotic portal bleeding in this situation. It represents a helpful option in the field of LT, when vascular bleeding cannot be stopped by other methods.