Biomarker discovery in asthma-related inflammation and remodeling

Proteomics. 2009 Apr;9(8):2163-70. doi: 10.1002/pmic.200800643.

Abstract

Asthma is a complex inflammatory disease of airways. A network of reciprocal interactions between inflammatory cells, peptidic mediators, extracellular matrix components, and proteases is thought to be involved in the installation and maintenance of asthma-related airway inflammation and remodeling. To date, new proteic mediators displaying significant activity in the pathophysiology of asthma are still to be unveiled. The main objective of this study was to uncover potential target proteins by using surface-enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS) on lung samples from mouse models of allergen-induced airway inflammation and remodeling. In this model, we pointed out several protein or peptide peaks that were preferentially expressed in diseased mice as compared to controls. We report the identification of different five proteins: found inflammatory zone 1 or RELM alpha (FIZZ-1), calcyclin (S100A6), clara cell secretory protein 10 (CC10), Ubiquitin, and Histone H4.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Asthma / metabolism*
  • Asthma / physiopathology
  • Biomarkers / metabolism*
  • Bronchi / chemistry
  • Bronchi / metabolism*
  • Bronchi / physiopathology
  • Cell Cycle Proteins / metabolism
  • Histones / metabolism
  • Inflammation / metabolism*
  • Inflammation / physiopathology
  • Intercellular Signaling Peptides and Proteins / metabolism
  • Mass Spectrometry
  • Mice
  • Protein Array Analysis
  • S100 Calcium Binding Protein A6
  • S100 Proteins / metabolism
  • Ubiquitin / metabolism
  • Uteroglobin / metabolism

Substances

  • Biomarkers
  • Cell Cycle Proteins
  • Histones
  • Intercellular Signaling Peptides and Proteins
  • Retnla protein, mouse
  • S100 Calcium Binding Protein A6
  • S100 Proteins
  • S100a6 protein, mouse
  • Scgb1a1 protein, mouse
  • Ubiquitin
  • Uteroglobin