Abstract
We previously reported that flavone induces embryonic lethality in Caenorhabditis elegans, which appeared to be the result of cell cycle arrest during early embryogenesis. To test this possibility, here we examined whether flavone inhibits the activity of a key cell cycle regulator, CDC-25.1 in C. elegans. A gain-of-function cdc-25.1 mutant, rr31, which exhibits extra cell divisions in intestinal cells, was used to test the inhibitory effects of flavone on CDC-25 activity. Flavone inhibited the extra cell divisions of intestinal cells in rr31, and modifications of flavone reduced the inhibitory effects. The inhibitory effects of flavone on CDC-25.1 were partly, if not completely, due to transcriptional repression.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Caenorhabditis elegans / cytology
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Caenorhabditis elegans / drug effects
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Caenorhabditis elegans / enzymology*
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Caenorhabditis elegans / genetics
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Caenorhabditis elegans Proteins / antagonists & inhibitors
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Caenorhabditis elegans Proteins / biosynthesis
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Caenorhabditis elegans Proteins / genetics
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Cell Cycle / drug effects
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Cell Cycle / physiology
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Flavones / pharmacology*
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Intestinal Mucosa / metabolism
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Intestines / cytology
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Intestines / drug effects
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cdc25 Phosphatases / antagonists & inhibitors*
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cdc25 Phosphatases / biosynthesis
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cdc25 Phosphatases / genetics
Substances
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Caenorhabditis elegans Proteins
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Flavones
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cdc25 Phosphatases