Analysis of bla(SHV) codon 238 and 240 allele mixtures using Sybr green high-resolution melting analysis

Patiyan Andersson; Tegan Harris; Steven Y C Tong; Philip M Giffard

doi:10.1128/AAC.00063-09

Analysis of bla(SHV) codon 238 and 240 allele mixtures using Sybr green high-resolution melting analysis

Antimicrob Agents Chemother. 2009 Jun;53(6):2679-83. doi: 10.1128/AAC.00063-09. Epub 2009 Apr 6.

Authors

Patiyan Andersson¹, Tegan Harris, Steven Y C Tong, Philip M Giffard

Affiliation

¹ Menzies School of Health Research, Institute of Advanced Studies, Charles Darwin University, Northern Territory 0810, Australia.

Abstract

Klebsiella pneumoniae isolates frequently contain complex mixtures of bla(SHV) alleles. A high-resolution melting-based method for interrogating the extended-spectrum activity conferring codon 238 and 240 polymorphisms was developed. This detects minority extended-spectrum beta-lactamase-encoding alleles, allows estimation of allele ratios, and discriminates between single and double mutants.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles
Benzothiazoles
Codon*
Diamines
Klebsiella pneumoniae / genetics
Organic Chemicals
Polymerase Chain Reaction
Polymorphism, Single Nucleotide
Quinolines
beta-Lactamases / genetics*

Substances

Benzothiazoles
Codon
Diamines
Organic Chemicals
Quinolines
SYBR Green I
beta-lactamase PIT-2
beta-lactamase SHV-11
beta-lactamase SHV-12
beta-lactamase SHV-2
beta-lactamase SHV-5
beta-Lactamases