A Saporin-6 cDNA containing a precursor sequence coding for a carboxyl-terminal extension

FEBS Lett. 1991 Oct 21;291(2):285-8. doi: 10.1016/0014-5793(91)81303-p.

Abstract

Saporin-6 is a single-chain ribosome inactivating protein (RIP) from the seeds and the leaves of Saponaria officinalis (Caryophyllaceae). Here we have identified the COOH-terminal end of mature Saporin-6 and, by cDNA sequencing, the predicted carboxyl-terminal sequence of a leaf Saporin-6 primary translation product. Our data indicate that the characterized cDNA codes for a precursor containing a 22 amino acid carboxyl-terminal extension, not present in mature Saporin-6, that shows similarity to carboxyl-terminal propeptides of vacuolar proteins, suggesting that it may be involved in protein trafficking.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA / chemistry*
  • Immunotoxins*
  • Molecular Sequence Data
  • N-Glycosyl Hydrolases*
  • Peptide Fragments / chemistry
  • Plant Proteins / chemistry
  • Plant Proteins / genetics*
  • Polymerase Chain Reaction
  • Protein Precursors / chemistry
  • Protein Precursors / genetics*
  • Ribosome Inactivating Proteins, Type 1
  • Saporins

Substances

  • Immunotoxins
  • Peptide Fragments
  • Plant Proteins
  • Protein Precursors
  • Ribosome Inactivating Proteins, Type 1
  • DNA
  • N-Glycosyl Hydrolases
  • Saporins

Associated data

  • GENBANK/D10379
  • GENBANK/D10380
  • GENBANK/D10381
  • GENBANK/D10382
  • GENBANK/D10383
  • GENBANK/D10384
  • GENBANK/S62474
  • GENBANK/S62510
  • GENBANK/X58803
  • GENBANK/X61394