Abstract
The expression of carbonic anhydrase IX (CAIX), a marker for hypoxic tumors, is correlated with poor prognosis in breast cancer patients. We show herein that the MDA-MB-231 cells, a "triple-negative," basal B line, express exclusively CAIX, while a luminal cell line (T47D) expresses carbonic anhydrase XII (CAXII). CAIX expression in the basal B cells is both density- and hypoxia-dependent and is correlated with carbonic anhydrase activity. Evidence is provided that CAIX contributes to extracellular acidification through studies on pH, lactic acid production, and CAIX inhibition. Together, these studies suggest that CAIX expression and activity is associated with metabolic dysfunction in MDA-MB-231 cells.
Publication types
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Comparative Study
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, Neoplasm / genetics
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Antigens, Neoplasm / metabolism*
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Breast Neoplasms / enzymology*
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Breast Neoplasms / genetics
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Breast Neoplasms / pathology
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Carbonic Anhydrase IX
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Carbonic Anhydrase Inhibitors / pharmacology
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Carbonic Anhydrases / genetics
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Carbonic Anhydrases / metabolism*
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Cell Hypoxia
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Cell Line, Tumor
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Cell Proliferation
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Female
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Gene Expression Regulation, Enzymologic
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Gene Expression Regulation, Neoplastic
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Glucose / metabolism
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Humans
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Hydrogen-Ion Concentration
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Lactic Acid / metabolism
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Phenotype
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RNA, Messenger / metabolism
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Time Factors
Substances
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Antigens, Neoplasm
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Carbonic Anhydrase Inhibitors
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RNA, Messenger
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Lactic Acid
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CA9 protein, human
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Carbonic Anhydrase IX
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Carbonic Anhydrases
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carbonic anhydrase XII
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Glucose