Abstract
A comprehensive analysis of the phosphoinositide interactome has been performed using analogues of PI(3,5)P2 and PI(4,5)P2 phosphatidyl phospholipids which were immobilized onto Affi-10 beads or incorporated into liposomes for use as affinity absorbents with cytosolic extracts from colonic carcinoma cell lines. Affinity/LC/MS/MS experiments allowed identification of 388 proteins/protein complexes that appeared to interact specifically with the phosphoinositide targets: a number of novel potential phosphoinositide interacting proteins have been identified.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Cell Line, Tumor
-
Chromatography, Ion Exchange / methods
-
Cytosol / metabolism
-
Electrophoresis, Polyacrylamide Gel
-
Gene Expression Regulation, Enzymologic*
-
Humans
-
Liposomes / chemistry
-
Magnetic Resonance Spectroscopy
-
Peptides / chemistry
-
Phosphatidylinositol 4,5-Diphosphate / chemistry*
-
Phosphatidylinositol 4,5-Diphosphate / metabolism
-
Phosphatidylinositol Phosphates / chemistry*
-
Phosphatidylinositol Phosphates / metabolism
-
Phospholipids / chemistry
-
Phosphorylation
-
Proteome
-
Proteomics / methods*
Substances
-
Liposomes
-
Peptides
-
Phosphatidylinositol 4,5-Diphosphate
-
Phosphatidylinositol Phosphates
-
Phospholipids
-
Proteome
-
phosphatidylinositol 3,5-diphosphate