First detection of plasmid-encoded blaOXY beta-lactamase

Antimicrob Agents Chemother. 2009 Jul;53(7):3143-6. doi: 10.1128/AAC.01473-08. Epub 2009 Apr 20.

Abstract

Three Klebsiella oxytoca isolates and one Klebsiella pneumoniae isolate from three children admitted to the Hematology Unit of Hospital Vall d'Hebron (Barcelona, Spain) exhibited a susceptibility pattern suggesting OXY beta-lactamase hyperproduction. All the isolates contained a 95-kb plasmid that harbored bla(OXY-1), which was transferred by electrotransformation but could not be self-transferred by conjugation. A qnrS1 gene was also harbored in the bla(OXY-1)-carrying plasmid. This is the first report of a plasmid-encoded OXY beta-lactamase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Southern
  • Child, Preschool
  • Drug Resistance, Bacterial / genetics
  • Electrophoresis, Gel, Pulsed-Field
  • Humans
  • Klebsiella / enzymology*
  • Klebsiella / genetics*
  • Klebsiella oxytoca / drug effects
  • Klebsiella oxytoca / genetics
  • Klebsiella pneumoniae / drug effects
  • Klebsiella pneumoniae / genetics
  • Male
  • Microbial Sensitivity Tests
  • Plasmids / genetics*
  • Polymerase Chain Reaction
  • beta-Lactamases / genetics*

Substances

  • beta-Lactamases