Enrichment broth improved detection of extended-spectrum-beta-lactamase-producing bacteria in throat and rectal surveillance cultures of samples from patients in intensive care units

Jean-Luc A N Murk; Edou R Heddema; Dorine L J Hess; Johannes A Bogaards; Christina M J E Vandenbroucke-Grauls; Yvette J Debets-Ossenkopp

doi:10.1128/JCM.01406-08

Enrichment broth improved detection of extended-spectrum-beta-lactamase-producing bacteria in throat and rectal surveillance cultures of samples from patients in intensive care units

J Clin Microbiol. 2009 Jun;47(6):1885-7. doi: 10.1128/JCM.01406-08. Epub 2009 Apr 22.

Authors

Jean-Luc A N Murk¹, Edou R Heddema, Dorine L J Hess, Johannes A Bogaards, Christina M J E Vandenbroucke-Grauls, Yvette J Debets-Ossenkopp

Affiliation

¹ VU University Medical Center, Medical Microbiology and Infection Control, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands. [email protected]

Abstract

We evaluated the use of a trypticase soy broth (TSB) for improving detection of extended-spectrum-beta-lactamase-producing (ESBL(+)) bacteria. Preenrichment of throat and rectal swabs in TSB prior to inoculation on solid medium doubled the number of ESBL(+) bacteria detected in samples obtained from patients in our intensive care unit.

Publication types

Evaluation Study

MeSH terms

Adult
Bacteria / enzymology*
Bacteria / isolation & purification*
Bacteriological Techniques / methods*
Humans
Intensive Care Units
Mass Screening / methods*
Pharynx / microbiology*
Rectum / microbiology*
Sensitivity and Specificity
beta-Lactamases / biosynthesis*

Substances

beta-Lactamases