Time-dependent variations in ischemia-modified albumin levels in mesenteric ischemia

Acad Emerg Med. 2009 Jun;16(6):539-43. doi: 10.1111/j.1553-2712.2009.00414.x. Epub 2009 Apr 21.

Abstract

Objectives: The objective was to determine the value of ischemia-modified albumin (IMA) in the diagnosis of mesenteric embolism. The authors investigated whether or not plasma IMA levels rose in the acute period in a rat model of mesenteric ischemia and the related time-dependent changes.

Methods: In this randomized, controlled, nonblinded trial, 36 mature female Wistar rats were divided into six groups: three control (Groups I, III, and V) and three ischemia (Groups II, IV, and VI). In the control groups, blood was sampled at 30 minutes (Group I), 2 hours (Group III), and 6 hours (Group V) following a simple laparotomy. In the ischemia groups, following laparotomy, the superior mesenteric artery (SMA) was clamped using a bulldog clamp, and blood samples were taken at 30 minutes (Group II), 2 hours (Group IV), and 6 hours (Group VI).

Results: Plasma IMA levels in the ischemia groups were significantly higher compared to those of the control groups (p < 0.004). In addition, levels were higher in the 6-hour blood samples of the ischemia group than in the 2-hour and 30-minute samples (p < 0.001). Serum IMA was also higher in the 2-hour blood samples of the ischemia group than in the 30-minute samples (p < 0.001).

Conclusions: These preliminary findings suggest that serum IMA levels may represent a significant parameter in the early diagnosis of acute mesenteric ischemia and that further studies are necessary.

MeSH terms

  • Animals
  • Constriction
  • Disease Models, Animal
  • Female
  • Intestine, Small / pathology
  • Ischemia / blood
  • Ischemia / diagnosis*
  • Lactic Acid / analysis
  • Malondialdehyde / analysis
  • Mesenteric Artery, Superior
  • Mesentery / blood supply*
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Serum Albumin / metabolism*
  • Statistics, Nonparametric
  • Time Factors

Substances

  • Serum Albumin
  • Lactic Acid
  • Malondialdehyde