Genomic and biochemical analysis of N glycosylation in the mushroom-forming basidiomycete Schizophyllum commune

Elsa Berends; Robin A Ohm; Jan F de Jong; Gerard Rouwendal; Han A B Wösten; Luis G Lugones; Dirk Bosch

doi:10.1128/AEM.00352-09

Genomic and biochemical analysis of N glycosylation in the mushroom-forming basidiomycete Schizophyllum commune

Appl Environ Microbiol. 2009 Jul;75(13):4648-52. doi: 10.1128/AEM.00352-09. Epub 2009 May 1.

Authors

Elsa Berends¹, Robin A Ohm, Jan F de Jong, Gerard Rouwendal, Han A B Wösten, Luis G Lugones, Dirk Bosch

Affiliation

¹ Molecular Microbiology and Kluyver Centre for Genomics of Industrial Fermentations, Institute of Biomembranes, Utrecht University, The Netherlands. [email protected]

Abstract

N-linked glycans of Schizophyllum commune consist of Man(5-9)GlcNAc(2) structures. Lack of further glycan maturation is explained by the absence of genes encoding such functions in this and other homobasidiomycetes. N-linked glycans in vegetative mycelium and fruiting bodies of S. commune are mainly Man(7)GlcNAc(2) and Man(5)GlcNAc(2), respectively, suggesting more efficient mannose trimming in the mushroom.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Genes, Fungal*
Glycosylation*
Humans
Mannose / metabolism
Models, Biological
Nitrogen / metabolism
Schizophyllum / genetics
Schizophyllum / metabolism*

Substances

Nitrogen
Mannose