Confocal light scattering spectroscopic imaging system for in situ tissue characterization

Appl Opt. 2009 May 1;48(13):2595-9. doi: 10.1364/ao.48.002595.

Abstract

We report on the design and construction of a confocal light scattering spectroscopic imaging system aimed ultimately to conduct depth-resolved characterization of biological tissues. The confocal sectioning ability of the system is demonstrated using a two-layer sample consisting of a 200 microm thick cancer cell layer on top of a scattering layer doped with a green absorber. The measurement results demonstrate that distinct light scattering signals can be isolated from each layer with an axial and a lateral resolution of 30 and 27 microm, respectively. Such a system is expected to have significant applications in the areas of tissue engineering and disease diagnostics and monitoring.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Cell Line, Tumor
  • Equipment Design
  • Equipment Failure Analysis
  • Humans
  • Image Enhancement / instrumentation*
  • Leukemia / pathology*
  • Leukemia / physiopathology*
  • Microscopy, Confocal / instrumentation*
  • Microscopy, Confocal / methods
  • Nephelometry and Turbidimetry / instrumentation*
  • Nephelometry and Turbidimetry / methods
  • Spectrum Analysis / instrumentation*
  • Spectrum Analysis / methods
  • Tomography, Optical Coherence / instrumentation*
  • Tomography, Optical Coherence / methods