We investigated the ability of blue-on-yellow perimetry (B/ YP) to identify the visual field loss in pituitary tumor (PT). All subjects underwent white-on-white perimetry (W/WP) and B/YP tests with Humphrey field analyzer 750i, program 30-2, full threshold. The efforts to detect visual field loss in PT patients with the chiasmal compression could be improved using B/YP in clinical practice.