High expression of WT1 gene in acute myeloid leukemias with more predominant WT1+17AA isoforms at relapse

Weiying Gu; Shaoyan Hu; Zixing Chen; Guoqiang Qiu; Jiannong Cen; Bai He; Jun He; Wei Wu

doi:10.1016/j.leukres.2009.04.004

High expression of WT1 gene in acute myeloid leukemias with more predominant WT1+17AA isoforms at relapse

Leuk Res. 2010 Jan;34(1):46-9. doi: 10.1016/j.leukres.2009.04.004. Epub 2009 May 2.

Authors

Weiying Gu¹, Shaoyan Hu, Zixing Chen, Guoqiang Qiu, Jiannong Cen, Bai He, Jun He, Wei Wu

Affiliation

¹ Department of Hematology, The First People's Hospital of Changzhou, Third Affiliated to Suzhou University, Changzhou, China. [email protected]

PMID: 19414192
DOI: 10.1016/j.leukres.2009.04.004

Abstract

Real-time quantitative reverse transcriptase polymerase chain reaction method was established for detecting the expression levels of WT1 gene and WT1+17AA isoforms in 226 acute myeloid leukemia (AML) bone marrow (BM) cells. The results showed that WT1 gene was 2-3 logarithms expressed more in AML BM cells at initial diagnosis or relapse than in normal BM cells (p<0.001), with predominant WT1+17AA isoforms expression (the ratio of WT1+17AA/WT1 more than 0.50). Interestingly the ratio of WT1+17AA/WT1 was statistically higher in relapsed AMLs than in initially diagnosed (p=0.01), speculating that WT1+17AA isoforms might participate in AML relapse.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
DNA Primers
Genes, Wilms Tumor*
Humans
Leukemia, Myeloid, Acute / genetics*
Leukemia, Myeloid, Acute / pathology
Recurrence
Reverse Transcriptase Polymerase Chain Reaction

Substances

DNA Primers