Characterization of the RNA-binding properties of the triple-gene-block protein 2 of Bamboo mosaic virus

Virol J. 2009 May 7:6:50. doi: 10.1186/1743-422X-6-50.

Abstract

The triple-gene-block protein 2 (TGBp2) of Bamboo mosaic virus (BaMV) is a transmembrane protein which was proposed to be involved in viral RNA binding during virus transport. Here, we report on the RNA-binding properties of TGBp2. Using tyrosine fluorescence spectroscopy and UV-crosslinking assays, the TGBp2 solubilized with Triton X-100 was found to interact with viral RNA in a non-specific manner. These results raise the possibility that TGBp2 facilitates intracellular delivery of viral RNA through non-specific protein-RNA interaction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Molecular Sequence Data
  • Potexvirus / physiology*
  • RNA, Viral / metabolism*
  • RNA-Binding Proteins / metabolism*
  • Sasa / virology*
  • Viral Proteins / metabolism*

Substances

  • RNA, Viral
  • RNA-Binding Proteins
  • Viral Proteins