Abstract
Cyclooxygenase-2 (COX-2) has been found to be over-expressed in esophageal carcinoma (EC) and it could be considered as a potential tumor-associated antigen (TAA). In the present study, six candidate peptides from COX-2 were firstly predicted and synthesized. Among them, P(479) had the highest affinity and stability toward both HLA-A *0201 and HLA-A *03 molecules and it could significantly promote the IFN-gamma release. The cytotoxic T lymphocytes (CTLs) induced by P(479) could specifically lyse COX-2-expressed EC cell lines, EC-1 (HLA-A3 supertype) and EC-9706 (HLA-A2 supertype). These results suggested that P(479) as a novel broad-spectrum T cell epitope would be very useful in immunotherapy against esophageal carcinoma.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, Neoplasm / biosynthesis*
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Antigens, Neoplasm / immunology
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CD8-Positive T-Lymphocytes / immunology*
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Cell Line, Tumor
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Cyclooxygenase 2 / biosynthesis*
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Cyclooxygenase 2 / immunology
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Cytotoxicity, Immunologic*
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Epitopes, T-Lymphocyte
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Esophageal Neoplasms / immunology*
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Esophageal Neoplasms / pathology
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HLA-A Antigens / immunology
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HLA-A2 Antigen
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HLA-A3 Antigen
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Humans
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Interferon-gamma / biosynthesis
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Oligopeptides / chemical synthesis
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Oligopeptides / immunology
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Oligopeptides / pharmacology*
Substances
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Antigens, Neoplasm
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Epitopes, T-Lymphocyte
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HLA-A Antigens
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HLA-A*02:01 antigen
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HLA-A*03 antigen
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HLA-A2 Antigen
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HLA-A3 Antigen
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Oligopeptides
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Interferon-gamma
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Cyclooxygenase 2