Protection in mice immunized with a heterologous prime-boost regime using DNA and recombinant pseudorabies expressing TgSAG1 against Toxoplasma gondii challenge

Limin Shang; Quan Liu; Wensen Liu; Jingtao Men; Shengyan Gao; Li Jiang; Ze Wang; Yujia Zhai; Hongtao Jin; Hai Lian; Chen Chen; Zhiping Xia; Ziguo Yuan; Xing-Quan Zhu

doi:10.1016/j.vaccine.2009.03.013

Protection in mice immunized with a heterologous prime-boost regime using DNA and recombinant pseudorabies expressing TgSAG1 against Toxoplasma gondii challenge

Vaccine. 2009 May 11;27(21):2741-5. doi: 10.1016/j.vaccine.2009.03.013. Epub 2009 Mar 25.

Authors

Limin Shang¹, Quan Liu, Wensen Liu, Jingtao Men, Shengyan Gao, Li Jiang, Ze Wang, Yujia Zhai, Hongtao Jin, Hai Lian, Chen Chen, Zhiping Xia, Ziguo Yuan, Xing-Quan Zhu

Affiliation

¹ Laboratory of Parasitology, Veterinary Institute, Academy of Military Medical Sciences, Changchun 130062, Jilin Province, China.

PMID: 19428887
DOI: 10.1016/j.vaccine.2009.03.013

Abstract

An effective vaccine of animals can block transmission of Toxoplasma gondii to humans. In this study, mice have been protected against lethal T. gondii challenge by a prime-boost vaccination strategy using DNA vaccine pVAX/TgSAG1 and recombinant pseudorabies virus rPRV/TgSAG1, both expressing the major immunodominant surface antigen of T. gondii (TgSAG1). High levels of splenocyte proliferative responses and significant levels of IFN-gamma resulted, with strong cytotoxic T lymphocyte (CTL) responses in vitro. After lethal challenge, prime-boost vaccinated mice showed an increased survival time (15.4+/-5.0 days) and a 40% survival rate compared with controls who all died within 11 days of challenge. Results of the present study indicated that this novel immunization strategy is useful in enhancing immune protection in mice against lethal T. gondii infection, which would provide foundation for the development of effective vaccines against T. gondii.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies / immunology
Antigens, Protozoan / genetics
Antigens, Protozoan / immunology*
Antigens, Protozoan / metabolism
Cell Proliferation
Cells, Cultured
Cytokines / immunology
Female
Herpesvirus 1, Suid / genetics
Herpesvirus 1, Suid / immunology*
Herpesvirus 1, Suid / metabolism
Immunization, Secondary / methods*
Mice
Mice, Inbred BALB C
Protozoan Proteins / genetics
Protozoan Proteins / immunology*
Protozoan Proteins / metabolism
Recombinant Proteins / genetics
Recombinant Proteins / immunology
Recombinant Proteins / metabolism
Spleen / immunology
Toxoplasma / genetics
Toxoplasma / immunology*
Toxoplasma / metabolism
Toxoplasmosis / immunology
Toxoplasmosis / parasitology
Toxoplasmosis / prevention & control*
Vaccines, DNA / genetics*
Vaccines, DNA / immunology*
Vaccines, DNA / metabolism

Substances

Antibodies
Antigens, Protozoan
Cytokines
Protozoan Proteins
Recombinant Proteins
SAG1 antigen, Toxoplasma
Vaccines, DNA