In vivo arginine production and nitric oxide synthesis in pregnant Indian women with normal and low body mass indices

Eur J Clin Nutr. 2009 Sep;63(9):1091-7. doi: 10.1038/ejcn.2009.24. Epub 2009 May 13.

Abstract

Background/objectives: Nitric oxide (NO) has been proposed as a mediator of vascular expansion during pregnancy. Inability to increase NO synthesis and/or production of its precursor, arginine, may be a contributor to pregnancy-induced hypertension or preeclampsia. Because maternal weight is associated with blood pressure and risk of preeclampsia during pregnancy, it may also influence arginine and/or NO production. The purpose of this study was to determine the in vivo arginine production and NO synthesis rate in pregnant women with normal (n=10) and low (n=10) body mass indices (BMIs).

Subjects/methods: Arginine flux and NO synthesis rate were measured in the postabsorptive state with constant infusions of 15N2-arginine and 13C,2H4-citrulline. Plasma concentrations of arginine and NO metabolites were also measured. Kinetic parameters were correlated to maternal variables, gestational age, birth weight and blood pressure.

Results: Endogenous arginine flux was significantly faster in the low-BMI compared with normal-BMI women in the first trimester (63.1+/-3.4 vs 50.2+/-2.0 micromol/kg per h, P<0.01), but not in the second. Plasma NO concentration was higher (44.7+/-5.3 vs 30.4+/-1.9 micromol/l, P=0.03) and its rate of synthesis trended faster in the low-BMI compared with normal-BMI group in the second trimester. Maternal weight and BMI were negatively correlated with arginine flux in both trimesters and NO synthesis in the second trimester.

Conclusions: These findings suggest, but do not prove, that maternal BMI may be a factor in the ability to produce NO during pregnancy and may be one way by which BMI influences blood pressure during pregnancy.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adolescent
  • Adult
  • Arginine / blood*
  • Body Mass Index*
  • Body Weight / physiology*
  • Female
  • Humans
  • India
  • Nitric Oxide / biosynthesis*
  • Nitric Oxide / blood
  • Overweight / blood
  • Overweight / complications*
  • Pregnancy / blood
  • Pregnancy / metabolism*
  • Pregnancy Complications / blood*
  • Pregnancy Trimester, First / blood
  • Pregnancy Trimester, Second / blood
  • Reference Values
  • Young Adult

Substances

  • Nitric Oxide
  • Arginine