[Study on inhibitive effect of extracts from Scindapsus aureus on alpha-glucosidase and its antioxidant activity]

Zhong Yao Cai. 2009 Jan;32(1):89-92.
[Article in Chinese]

Abstract

Objective: To study the inhibitive effect of extracts from Scindapsus aureus on alpha-glucosidase and its antioxidant activity.

Methods: The 70% ethanol extracts was extracted by petroleum, ethyl acetate and n-butanol, and the inhibitory activity against alpha-glucosidases and antioxidative effects of each fraction were determined in vitro, and the inhibitory kinetics of ethyl acetate fraction was investigated.

Results: The inhibitory activity of ethly acetate fraction against alpha-glucosidase was higher as its IC50 was 43.63 microg/mL. The inhibition kinetics analyzed by Hnewaver-Burk plots showed that ethyl acetate fraction was a competitive type inhibitor, and the inhibition constants for free ennzyme (Ki) was determined to be 21.49 microg/mL. The antioxidative effects of ethly acetate and n-butanol fractions exhibited stronger than that of other fractions, the scavenging ability on DPPH at the concentration of 0.42 mg/mL were 88.5% and 93.6%. Ethly acetate fraction showed scavenging ability on autioxidantor, and the IC50 values was 0.27 mg/mL.

Conclusion: Ethyld acetate fraction of extract from Scindapsus aureus has potential inhibitroy activity against alpha-glucosidase and antioxidant effects, and n-butanol fraction has great antioxidative effects.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetates
  • Antioxidants / isolation & purification
  • Antioxidants / pharmacology*
  • Araceae / chemistry*
  • Araceae / growth & development
  • Ethanol
  • Free Radicals / metabolism*
  • Glycoside Hydrolase Inhibitors*
  • Inhibitory Concentration 50
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Plants, Medicinal / chemistry
  • Plants, Medicinal / growth & development
  • Spectrophotometry, Ultraviolet
  • alpha-Glucosidases / metabolism

Substances

  • Acetates
  • Antioxidants
  • Free Radicals
  • Glycoside Hydrolase Inhibitors
  • Plant Extracts
  • Ethanol
  • ethyl acetate
  • alpha-Glucosidases