Using HLB cartridge for extraction and cleanup, a method for simultaneous determination of 4 tetracycline and 6 quinolone antibiotics in environmental water samples was developed by ultra pressure liquid chromatography coupled with tandem mass spectrometry. Using river water and sea water as matrices and carbadox as surrogate, the recoveries and relative standard deviations (RSD, n = 4) were 94.0% - 117.0% and 2.0% - 9.7% for 4 tetracyclines at 20.0 ng/L and 100.0 ng/L spiking levels and 63.6% - 93.9% and 1.6% - 8.1% for 6 quinolones at 5.0 ng/L and 20.0 ng/L spiking levels, respectively. The detection limits were 20.0 ng/L for 4 tetracyclines and 0.4 ng/L for 6 quinolones. The method has been successfully applied to the survey of 10 target antibiotic residues in Jiulong River estuary, Fujian.