Development of a pUC19-based recombinant plasmid to serve as a positive control in PCR for Orientia tsutsugamushi

Nozomu Hanaoka; Akiko Sakata; Ai Takano; Hiroki Kawabata; Haruo Watanabe; Ichiro Kurane; Toshio Kishimoto; Shuji Ando

doi:10.1111/j.1348-0421.2009.00126.x

Development of a pUC19-based recombinant plasmid to serve as a positive control in PCR for Orientia tsutsugamushi

Microbiol Immunol. 2009 May;53(5):305-8. doi: 10.1111/j.1348-0421.2009.00126.x. Epub 2009 Feb 18.

Authors

Nozomu Hanaoka¹, Akiko Sakata, Ai Takano, Hiroki Kawabata, Haruo Watanabe, Ichiro Kurane, Toshio Kishimoto, Shuji Ando

Affiliation

¹ Department of Virology-I, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan.

PMID: 19457173
DOI: 10.1111/j.1348-0421.2009.00126.x

Abstract

A recombinant positive control plasmid was developed to be used in PCR for Orientia tsutsugamushi. This pUC19-based plasmid contains the O. tsutsugamushi DNA sequence, part of which is replaced with Candida albicans DNA. pUC19-Posi is a PCR control that provides several advantages over currently used positive controls for both universal and serotypical PCR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Candida albicans / genetics
DNA, Recombinant / genetics*
Orientia tsutsugamushi / genetics*
Plasmids / genetics*
Polymerase Chain Reaction / methods
Polymerase Chain Reaction / standards*
Reference Standards

Substances

DNA, Recombinant