Partial atlE sequencing of Staphylococcus epidermidis strains from prosthetic joint infections

J Clin Microbiol. 2009 Jul;47(7):2321-4. doi: 10.1128/JCM.01971-08. Epub 2009 May 20.

Abstract

Partial atlE sequencing (atlE nucleotides 2782 to 3114 [atlE(2782-3114)]) was performed in 41 Staphylococcus epidermidis isolates from prosthetic joint infections (PJIs) and 44 isolates from skin as controls. The atlE(2782-3114) allele 1 (type strain sequence) was significantly more frequent in PJI strains (38/41 versus 29/44 in controls; P = 0.0023). Most PJI strains were positive for mecA, icaA/icaD, and IS256, and most belonged to the sequence type 27 subgroup, suggesting the involvement of few related clones.

MeSH terms

  • Alleles
  • Bacterial Proteins / genetics*
  • Bacterial Typing Techniques
  • Cluster Analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Genotype
  • Humans
  • Joint Diseases / microbiology*
  • Molecular Sequence Data
  • Prosthesis-Related Infections / microbiology*
  • Sequence Analysis, DNA
  • Staphylococcal Infections / microbiology*
  • Staphylococcus epidermidis / classification*
  • Staphylococcus epidermidis / genetics*
  • Staphylococcus epidermidis / isolation & purification
  • Virulence Factors / genetics

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • Virulence Factors

Associated data

  • GENBANK/FJ009419
  • GENBANK/FJ009420
  • GENBANK/FJ009421
  • GENBANK/FJ009422
  • GENBANK/FJ009423
  • GENBANK/FJ009424
  • GENBANK/FJ009425
  • GENBANK/FJ009426
  • GENBANK/FJ009427
  • GENBANK/FJ009428