[Molecular cloning and characterization of Ras-homologues from different developing stages of Echinococcus granulosus]

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2009 Feb 28;27(1):91-3.
[Article in Chinese]

Abstract

The Ras GTPase gene from protoscolex and adult worm of Echinococcus granulosus in Xinjiang were cloned by RT-PCR and named as Eg Ras-pro (GenBank No. EU560397) and Eg Ras-adult (GenBank No. EU560398). Sequence analysis showed that each gene had 552 bp, coding 184 aa with an pI of 6.54. Bioinformatics analysis revealed that Eg Ras-pro and Eg Ras-adult had 98.4% and 98.9% homology to Echinococcus multilocularis Ras and 53.9%-78.8% homology to other species. Phylogenetic analysis showed that Eg Ras-pro and Eg Ras-adult clustered with EmRas and SmRas. The data indicated that EgRas GTPase has been expressed from protoscolex and adult worm of Echinococcus granulosus, and both are highly conservative.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cloning, Molecular
  • Echinococcus granulosus / enzymology
  • Echinococcus granulosus / genetics*
  • Helminth Proteins / genetics*
  • Monomeric GTP-Binding Proteins / genetics*
  • Phylogeny
  • Sequence Analysis, DNA

Substances

  • Helminth Proteins
  • Monomeric GTP-Binding Proteins