Plasma PAF-acetylhydrolase activity, inflammatory markers and susceptibility of LDL to in vitro oxidation in patients with type 1 diabetes mellitus

Diabetes Res Clin Pract. 2009 Jul;85(1):61-8. doi: 10.1016/j.diabres.2009.04.016. Epub 2009 May 22.

Abstract

Aims: We evaluated if type 1 diabetic patients have altered levels of low-grade inflammatory markers including platelet-activating factor acetylhydrolase (PAF-AH) activity, high-sensitivity C-reactive protein (hs-CRP), white blood cells count (WBC), erythrocyte sedimentation rate (ESR) and LDL-cholesterol oxidation coefficient (OxC), and whether their levels are related to clinical complications and the metabolic profile.

Methods: We studied cross-sectionally 118 outpatients with type 1 diabetes both with (n=38) and without (n=80) clinical chronic complications, and 110 controls subjects.

Results: Diabetic patients had higher levels of PAF-AH activity (p=0.02), hs-CRP (p=0.001), WBC (p=0.004) and ESR (p=0.0001) than controls. Patients with type 1 diabetes had earlier OxC than the non-diabetic subjects at 1 and 3 h (p=0.01) after the addition of CuSO(4). No difference was noted between patients with and without chronic complications. In the patients, correlations were found between PAF-AH activity and OxC at 1 and 3 h.

Conclusions: It was concluded that the susceptibility of LDL to in vitro oxidation and low-grade inflammation are increased in patients with type 1 diabetes and related to each other. The presence of these markers is probably independent of the presence of chronic clinical complications and reveals the development of early pro-inflammatory state.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-Alkyl-2-acetylglycerophosphocholine Esterase / blood*
  • Adolescent
  • Adult
  • Biomarkers / blood
  • C-Reactive Protein / metabolism
  • Cholesterol, LDL / blood
  • Cross-Sectional Studies
  • Diabetes Mellitus, Type 1 / blood*
  • Diabetes Mellitus, Type 1 / enzymology*
  • Female
  • Humans
  • Inflammation / blood*
  • Leukocyte Count
  • Male
  • Middle Aged
  • Outpatients
  • Oxidation-Reduction
  • Reference Values
  • Surveys and Questionnaires
  • Young Adult

Substances

  • Biomarkers
  • Cholesterol, LDL
  • C-Reactive Protein
  • 1-Alkyl-2-acetylglycerophosphocholine Esterase