Micro-CT in drug delivery

Eur J Pharm Biopharm. 2010 Jan;74(1):41-9. doi: 10.1016/j.ejpb.2009.05.008. Epub 2009 May 22.

Abstract

Micro-computed tomography (micro-CT) has not to date been fully exploited in the area of controlled drug delivery despite its capability for providing detailed, 3-D images of morphology and the opportunity this presents for exploring the relationships between delivery device formulation, structure and performance. Micro-CT was used to characterize the internal structure of polycaprolactone (PCL) matrix-type devices incorporating soluble particulates (lactose Mw 342.30, gelatin Mw 20-25kDa) as models of hydrophilic bioactives or pore-forming excipients. Micro-CT images confirmed that the lactose and gelatin particles were uniformly dispersed throughout the PCL phase and that efficient delivery of 95-100% of each species in 9days involved transport from the matrix core. Quantitative analysis of micro-CT images provided values for matrix macroporosity, which were within 15% of the theoretical value and revealed uniform porosity throughout the samples. Total release of protein occurred in 9days (PBS, 37 degrees C) from matrices containing a high protein load (44%w/w) and was independent of particle size. Measurements of equivalent pore diameter and frequency distribution identified a large population of sub-40microm pores in each material, indicative of a high density of connecting channels between particles which facilitates protein transport through the matrices.

MeSH terms

  • Delayed-Action Preparations / chemistry*
  • Diffusion
  • Drug Delivery Systems / methods*
  • Excipients / analysis
  • Excipients / chemistry
  • Imaging, Three-Dimensional / methods
  • Pharmaceutical Preparations / analysis
  • Pharmaceutical Preparations / chemistry
  • Polyesters / chemistry
  • Porosity
  • Proteins / analysis
  • Proteins / chemistry
  • Time Factors
  • X-Ray Microtomography / methods*

Substances

  • Delayed-Action Preparations
  • Excipients
  • Pharmaceutical Preparations
  • Polyesters
  • Proteins
  • polycaprolactone