Abstract
Dendritic cells can prime naive CD4+ T cells; however, here we demonstrate that dendritic cell-mediated priming was insufficient for the development of T helper type 2 cell-dependent immunity. We identify basophils as a dominant cell population that coexpressed major histocompatibility complex class II and interleukin 4 message after helminth infection. Basophilia was promoted by thymic stromal lymphopoietin, and depletion of basophils impaired immunity to helminth infection. Basophils promoted antigen-specific CD4+ T cell proliferation and interleukin 4 production in vitro, and transfer of basophils augmented the population expansion of helminth-responsive CD4+ T cells in vivo. Collectively, our studies suggest that major histocompatibility complex class II-dependent interactions between basophils and CD4+ T cells promote T helper type 2 cytokine responses and immunity to helminth infection.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Basophils / cytology
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Basophils / immunology*
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Basophils / metabolism
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CD11c Antigen / immunology
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CD4-Positive T-Lymphocytes / cytology
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CD4-Positive T-Lymphocytes / immunology*
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CD4-Positive T-Lymphocytes / metabolism
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Cell Communication / immunology
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Cell Proliferation
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Cytokines / immunology*
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Cytokines / metabolism
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Female
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Flow Cytometry
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Gene Expression
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Histocompatibility Antigens Class II / genetics
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Histocompatibility Antigens Class II / immunology*
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Immunity / immunology*
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Immunoblotting
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Interleukin-4 / genetics
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Interleukin-4 / metabolism
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Male
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Mice
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Mice, Inbred C57BL
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Mice, Inbred Strains
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Mice, Knockout
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Reverse Transcriptase Polymerase Chain Reaction
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Schistosomiasis mansoni / immunology
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Schistosomiasis mansoni / parasitology
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Th2 Cells / metabolism
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Thymus Gland / cytology
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Thymus Gland / immunology
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Trichuriasis / immunology
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Trichuriasis / parasitology
Substances
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CD11c Antigen
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Cytokines
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Histocompatibility Antigens Class II
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Interleukin-4