Abstract
In this paper, we describe a rapid flow cytometry method to identify antimicrobial peptides that are internalized into bacterial cells and differentiate them from those that are membrane active. The method was applied to fluorescently labeled Bac7(1-35) and polymyxin B, whose mechanisms of action are, respectively, based on cell penetration and on membrane binding and permeabilization. Identification of peptides with the former mechanism is of considerable interest for the intracellular delivery of membrane-impermeant drugs.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Antimicrobial Cationic Peptides / chemistry*
-
Antimicrobial Cationic Peptides / pharmacokinetics*
-
Escherichia coli / drug effects
-
Escherichia coli / metabolism
-
Flow Cytometry
-
Gram-Negative Bacteria / drug effects
-
Gram-Negative Bacteria / metabolism*
-
Microscopy, Confocal
-
Peptides, Cyclic / chemistry
-
Peptides, Cyclic / pharmacokinetics
-
Polymyxin B / chemistry
-
Polymyxin B / pharmacokinetics
Substances
-
Antimicrobial Cationic Peptides
-
Peptides, Cyclic
-
bactenecin
-
Polymyxin B