Specific Arabidopsis HSP90.2 alleles recapitulate RAR1 cochaperone function in plant NB-LRR disease resistance protein regulation

Proc Natl Acad Sci U S A. 2009 Jun 16;106(24):9556-63. doi: 10.1073/pnas.0904877106. Epub 2009 Jun 1.

Abstract

Both plants and animals require the activity of proteins containing nucleotide binding (NB) domain and leucine-rich repeat (LRR) domains for proper immune system function. NB-LRR proteins in plants (NLR proteins in animals) also require conserved regulation via the proteins SGT1 and cytosolic HSP90. RAR1, a protein specifically required for plant innate immunity, interacts with SGT1 and HSP90 to maintain proper NB-LRR protein steady-state levels. Here, we present the identification and characterization of specific mutations in Arabidopsis HSP90.2 that suppress all known phenotypes of rar1. These mutations are unique with respect to the many mutant alleles of HSP90 identified in all systems in that they can bypass the requirement for a cochaperone and result in the recovery of client protein accumulation and function. Additionally, these mutations separate HSP90 ATP hydrolysis from HSP90 function in client protein folding and/or accumulation. By recapitulating the activity of RAR1, these novel hsp90 alleles allow us to propose that RAR1 regulates the physical open-close cycling of a known "lid structure" that is used as a dynamic regulatory HSP90 mechanism. Thus, in rar1, lid cycling is locked into a conformation favoring NB-LRR client degradation, likely via SGT1 and the proteasome.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Alleles*
  • Arabidopsis / genetics*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / physiology*
  • Carrier Proteins / genetics
  • Carrier Proteins / physiology*
  • HSP90 Heat-Shock Proteins / genetics*
  • Intracellular Signaling Peptides and Proteins
  • Plant Diseases / genetics*

Substances

  • Arabidopsis Proteins
  • Carrier Proteins
  • HSP90 Heat-Shock Proteins
  • Intracellular Signaling Peptides and Proteins
  • PBS2 protein, Arabidopsis